Effect of Bovine Serum
نویسندگان
چکیده
The objectives of this s tudy were to test the effects of four levels of bovine serum albumin (BSA) on the development of ovine embryos cultured in Brinster's and Whitten's media and to examine the extent of in vitro development of ovine embryos cultured from various cell stages. Two hundred and forty-three oneto 16-celi ovine embryos were cultured in Brinster's medium (BMOC-2 plus .1% w/v dextrose) or Whitten's medium (WM) supplemented with one of four concentrations (.1, .5, 1.5 or 2.0%) of BSA. Embryos were grouped according to cell stage (oneto four-cell, fiveto eight-cell and nine-to 16-cetl) at outset of culture. In vitro development was based on completion of two cleavages and(or) development into a blastocyst. Percentages of embryos developing in culture were analyzed by least-square means for effects of media, BSA concentrations and cell stage at outset of culture. No differences in rate of development were observed between BMOC-2 and WM at any of the four BSA concentrations or at any of the three beginning developmental stages. Media with high BSA levels (1.5 and 2.0%) were superior (P<.01) to those with low BSA levels (.1 and .5%) in supporting two cleavages at all three stages. In media with low BSA concentrations (.1 and .5%), two or more cleavages occurred in 42, 40, and 63% of the oneto four-cell, fiveto eight-cell and nineto 16-cell embryos, while in media with high BSA concentrations (1.5 and
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